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In vitro og in vivo modeller til at studere hornhindeendothel-mesenkymale Transition
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
In Vitro and In Vivo Models to Study Corneal Endothelial-mesenchymal Transition
DOI:

09:05 min

August 20, 2016

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Chapters

  • 00:05Title
  • 01:06Primary Preparation and Immunostaining of Bovine Corneal Endothelial Cells
  • 04:24Rat Corneal Endothelium Cryoinjury Model and Intracameral Injection
  • 06:46Results: Immunostaining of In Vitro Cultured Bovine CECs with Marimastat Rat Corneal Buttons After Cryoinjury
  • 08:27Conclusion

Summary

Automatic Translation

En primær kultur af bovine hornhindeendothelceller blev anvendt til undersøgelse af mekanismen af ​​hornhindeendothel-mesenkymal overgang. Endvidere blev en rotte hornhindeendothel kryobeskadigelse model, der anvendes til at demonstrere hornhindeendothel-mesenchymal overgang in vivo.

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