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Sammenligning af Tobak Host Cell Protein Removal Metoder ved Blanchering Intakte Planter eller ved varmebehandling af ekstrakter
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Comparison of Tobacco Host Cell Protein Removal Methods by Blanching Intact Plants or by Heat Treatment of Extracts

Sammenligning af Tobak Host Cell Protein Removal Metoder ved Blanchering Intakte Planter eller ved varmebehandling af ekstrakter

DOI:
10.3791/54343-v

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11:06 min

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August 08, 2016

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, ,
1Fraunhofer Institute for Molecular Biology and Applied Ecology IME,Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e. V., 2Institute for Molecular Biotechnology,RWTH Aachen University, 3Department of Biomolecular Separation Engineering,Karlsruhe Institute of Technology (KIT)

Chapters

  • 00:05Title
  • 00:43Heat Precipitation of Tobacco Leaves by Blanching
  • 02:58Heat Precipitation in a Stirred Vessel
  • 04:53Heat Precipitation in a Heat Exchanger and Bag Filtration of Plant Extract
  • 07:55Results
  • 10:09Conclusion

Summary

Automatic Translation

Automatic Translation

Tre varme fældningsmetoder præsenteres som effektivt fjerner mere end 90% af værtscelleproteiner (HCPS) fra tobaksekstrakter forud for enhver anden oprensningstrin. Anlægget HCPS irreversibelt aggregerer ved temperaturer over 60 ° C.

Tags

Keywords: TobaccoHost Cell ProteinsHeat TreatmentBlanchingProtein RemovalPurificationRecombinant BiopharmaceuticalsProteasesExtractionWater BathTemperature ControlIncubation Time

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