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Utvikling av et innlegg Co-kultur System of Two Cellular Typer i fravær av Cell-Cell Kontakt
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JoVE Journal Biology
Development of an Insert Co-culture System of Two Cellular Types in the Absence of Cell-Cell Contact

Utvikling av et innlegg Co-kultur System of Two Cellular Typer i fravær av Cell-Cell Kontakt

DOI:
10.3791/54356-v

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11:29 min

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July 17, 2016

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,
1Dept. of Medical Biology,University of Québec

Chapters

  • 00:00Title
  • 01:54Seeding of N9 Microglia in Inserts
  • 03:47Activation of N9 Microglia With Lipopolysaccharide
  • 05:02Co-Cultivation of Neuronal PC12 Cells With N9 Microglia
  • 07:31Results: Lipopolysaccharide-activated N9 Microglia Secrete Cytokines That Are Toxic to Neuronal PC12 Cells
  • 09:48Conclusion

Summary

Automatic Translation

Automatic Translation

In multicellular organisms, secreted soluble factors elicit responses from different cell types as a result of paracrine signaling. Insert co-culture systems offer a simple way to assess the changes mediated by secreted soluble factors in the absence of cell-cell contact.

Tags

Keywords: Insert Co-culture SystemTwo Cellular TypesCell-cell ContactParacrine SignalingSecreted Soluble FactorsCell PolarityN9 MicrogliaPC12 CellsCytokine Toxicity

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