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Detection of Modified Forms of Cytosine Using Sensitive Immunohistochemistry
 

Detection of Modified Forms of Cytosine Using Sensitive Immunohistochemistry

Article DOI: 10.3791/54416-v • 09:08 min • August 16th, 2016
August 16th, 2016 •
1, 2, 3, 1, 4
1Laboratoire de Neurophysiologie (CP601), ULB Neuroscience Institute (UNI), Université Libre de Bruxelles, 2Medical Molecular Sciences, Centre for Biomolecular Sciences, University of Nottingham, 3School of Life Sciences, University of Nottingham, 4Division of Cancer and Stem Cells, Centre for Biomolecular Sciences, School of Medicine, University of Nottingham

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Summary
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Herein we describe a sensitive immunochemical method for mapping the spatial distribution of 5mC oxidation derivatives based on the use of peroxidase-conjugated secondary antibodies and tyramide signal amplification.

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Keywords: Modified Cytosine Immunohistochemistry Tissue Sections 5hmC 5caC Peroxidase Conjugated Secondary Antibodies Tyramide Signal Amplification Nuclear Localization Protein Lineage Markers DNA Depurination PFA FA PBX PBT HCl Tris-HCl Blocking Solution Mouse Monoclonal Anti-5hmC Rabbit Polyclonal Anti-5caC
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