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为葡萄糖和脂肪酸氧化率在大鼠离体的测定方法
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Methods for the Determination of Rates of Glucose and Fatty Acid Oxidation in the Isolated Working Rat Heart
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为葡萄糖和脂肪酸氧化率在大鼠离体的测定方法

DOI:
10.3791/54497-v

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12:24 min

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September 28, 2016

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, ,
1Department of Physiology and Biophysics, Mississippi Center for Obesity Research, Cardiovascular-Renal Research Center,University of Mississippi Medical Center

Chapters

  • 00:05Title
  • 01:07Rat Heart Isolation and Cannulation
  • 04:51Measurement of Cardiac Function and Sample Collection
  • 07:17Determination of Myocardial Glucose Oxidation Rate
  • 08:48Determination of Myocardial Oleate Oxidation Rates
  • 10:14Results: Measurements from Two Stressed Hearts
  • 11:40Conclusion

Summary

Automatic Translation

Automatic Translation

The following protocol describes the preparation and utilization of buffers for the quantitative measurement of rates of glucose and fatty acid oxidation in the isolated working rat heart. The methods used for sample analysis and data interpretation are also discussed.

Tags

Keywords: Glucose OxidationFatty Acid OxidationIsolated Working Rat HeartCardiac Contractual FunctionCardiovascularMetabolic AdaptationEnergy DemandsPathological ConditionsPerfusion ApparatusHeparinAortic ArchLangendorff Perfusion

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