Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / In Situ Labeling of Mitochondrial DNA Replication …
JoVE Journal
Genetics

This content is Open Access.

JoVE Journal Genetics
In Situ Labeling of Mitochondrial DNA Replication in Drosophila Adult Ovaries by EdU Staining
 

In Situ Labeling of Mitochondrial DNA Replication in Drosophila Adult Ovaries by EdU Staining

Article DOI: 10.3791/54516-v • 10:31 min • October 15th, 2016
October 15th, 2016 •
1, 1
1Lab of Molecular Genetics, National Heart, Lung and Blood Institute, National Institutes of Health

Chapters

Summary
Automatic Translation

Drosophila oogenesis continues to be exceptionally useful in the study of mitochondrial proliferation and inheritance. This manuscript describes a detailed protocol used to label the replicating mitochondrial DNA (mtDNA) in Drosophila adult ovaries with 5-ethynyl-2´-deoxyuridine (EdU), which facilitates uncovering mechanisms associated with mitochondrial inheritance that were previously debatable.

Tags

Keywords: Mitochondrial DNA Replication Drosophila Adult Ovaries EdU Staining Mitochondrial Biogenesis Mitochondrial DNA Inheritance In Situ Labeling Drosophila Medium Aphidicolin EdU Tissue Dissection Ovary Dissection Ovarioles Fluorescent Dye Penetration
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter