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The Preparation of Oblique Spinal Cord Slices for Ventral Root Stimulation

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

The Preparation of Oblique Spinal Cord Slices for Ventral Root Stimulation

The Preparation of Oblique Spinal Cord Slices for Ventral Root Stimulation

DOI:

10.3791/54525-v

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09:10 min

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October 13, 2016

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Félix Leroy, Boris Lamotte d’Incamps

¹Centre National de la Recherche Scientifique (UMR 8119),Centre de Neurophysique, Physiologie et Pathologie, Université Paris Descartes

Chapters

00:05Title
00:43Spinal Cord Dissection
02:41Agar Embedding
03:50Sectioning the Spinal Cord
05:02Preparation for Patch-Clamp Recording
06:40Results: Motoneuron Response to Ventral Root Stimulation
08:28Conclusion

Summary

Automatic Translation

We show how to prepare oblique slices of the spinal cord in young mice. This preparation allows for the stimulation of the ventral roots.

Tags

Keywords: Spinal Cord Slices Ventral Root Stimulation Motoneuron Identification Dissection Laminectomy Dura Removal Orientation Marking Agar Embedding

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