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Metoder til isolering, Kultur og funktionel karakterisering af sinoatrialt Node Myocytter fra voksne mus
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JoVE Journal Biology
Methods for the Isolation, Culture, and Functional Characterization of Sinoatrial Node Myocytes from Adult Mice

Metoder til isolering, Kultur og funktionel karakterisering af sinoatrialt Node Myocytter fra voksne mus

DOI:
10.3791/54555-v

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09:32 min

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October 23, 2016

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, ,
1Department of Physiology and Biophysics,University of Colorado Anschutz Medical Campus, 2Department of Bioengineering,University of Colorado Anschutz Medical Campus, 3Department of Medicine, Division of Cardiology,University of Colorado Anschutz Medical Campus

Chapters

  • 00:05Title
  • 00:44Sinoatrial Node Isolation
  • 04:25Sinoatrial Node Digestion and Sinoatrial Node Myocyte Dissociation
  • 06:29Sinoatrial Node Calcium Re-adaptation
  • 07:36Results: Electrophysiological Characterization of Cultured Sinoatrial Myocytes
  • 08:27Conclusion

Summary

Automatic Translation

Automatic Translation

Fremgangsmåder er påvist for isoleringen af ​​sinusknude myocytter (SAMS) fra voksne mus for patch clamp elektrofysiologi eller billeddannende undersøgelser. Isolerede celler kan anvendes direkte eller kan opretholdes i kultur for at tillade ekspression af proteiner af interesse, såsom genetisk indkodede reportere.

Tags

Sinoatrial Node MyocytesCardiac PacemakingElectrophysiologyCalcium ReleaseMouseHeart DissectionAtriumTyrode’s SolutionHeparin

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