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Vitronectin 在细菌黏附和血清耐药性中作用的实验研究
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Assays for Studying the Role of Vitronectin in Bacterial Adhesion and Serum Resistance
DOI:

10:55 min

October 16, 2018

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Chapters

  • 00:04Title
  • 00:49Detection of Vitronectin (Vn) Binding at the Bacterial Surface Using Flow Cytometry
  • 02:18ELISA Analysis of the Interaction Between Recombinant Protein H (PH) and Vn
  • 04:11Study of the Interaction Kinetics of Recombinant PH and Vn Using Bio-layer Interferometry
  • 04:33Bacterial Adherence to the Vn-coated Glass Surfaces
  • 05:32Vn-dependent Adherence of Bacteria to Epithelial Cells
  • 07:29Analysis of Vn-dependent Resistance to the Bactericidal Activity of Human Serum
  • 08:29Results: Interaction of Vitronectin and Bacteria
  • 10:10Conclusion

Summary

Automatic Translation

本报告描述了用于表征细菌外膜蛋白与人体补体调节器 vitronectin 之间相互作用的协议。该协议可用于研究 vitronectin 在任何细菌物种中的结合反应和生物学功能。

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