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Chapters
- 00:05Title
- 00:47Preparation of Media and Starter Cultures
- 01:54Co-inoculation of Fluorescently Labeled Strains for Surface Spreading
- 03:16Co-inoculation of Fluorescently Labeled Strains for Biofilm Growth
- 04:32Colony Imaging Using Fluorescent Microscopy
- 05:36Results: Swarming, Sliding, and Biofilm Producing Colonies Display Different Patternsof Spatial Growth
- 07:12Conclusion
Rolle sortiment (rumlig adskillelse) i evolutionære scenarier kan undersøges ved hjælp af simple mikrobielle systemer i laboratoriet, der tillader kontrolleret justering af rumlige fordeling. Ved at modificere grundlægger celletæthed kan forskellige sortiment niveauer visualiseres under anvendelse fluorescensmærkede bakteriestammer i kolonien biofilm af Bacillus subtilis.
Tags
Keywords: Spatial SegregationMicrobial PopulationsFluorescent MicroscopySwarmingSlidingBiofilm FormationSocial MicrobiologyFitnessImage AnalysisB. SubtilisLB MediumSwarming PlatesSliding PlatesColony BiofilmSG MediumGreen And Red Fluorescent ProteinOptical DensityMicroscopic TechniquesMicrobial Interaction
