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एक filamentous फफूंद कालोनियों पर बैक्टीरियल biofilms के गुणात्मक बहु पैमाने विश्लेषण के लिए नई विधि का उपयोग Confocal और इलेक्ट्रॉन माइक्रोस्कोपी
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
A New Method for Qualitative Multi-scale Analysis of Bacterial Biofilms on Filamentous Fungal Colonies Using Confocal and Electron Microscopy
DOI:

09:45 min

January 25, 2017

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Chapters

  • 00:05Title
  • 00:50Preparation of Bacterial Fungal Cultures
  • 02:11Preparation of In Vitro Biofilm of Bacteria on the Fungal Colony
  • 03:41Sample Preparation for Confocal Microscopy Analysis of Biofilm Formation
  • 05:35Confocal and Electron Microscopic Analysis
  • 07:07Results: Meso- and Micro-scale Analysis of P. fluorescens BBc6 on L. bicolor
  • 08:29Conclusion

Summary

Automatic Translation

This protocol describes a new method to grow and qualitatively analyze bacterial biofilms on fungal hyphae by confocal and electron microscopy.

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