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Preparation of Primary Mixed Glial Cultures from Adult Mouse Spinal Cord Tissue
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JoVE Journal Neuroscience
Preparation of Primary Mixed Glial Cultures from Adult Mouse Spinal Cord Tissue

Preparation of Primary Mixed Glial Cultures from Adult Mouse Spinal Cord Tissue

DOI:
10.3791/54801-v

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07:13 min

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November 19, 2016

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1Department of Biomedical Sciences, College of Osteopathic Medicine,University of New England

Chapters

  • 00:05Title
  • 00:55Preparation of the Single Cell Suspension
  • 03:12Removal of Myelin from the Single Cell Suspension
  • 05:03Results: Mixed Glial Cultures and Response to Stimuli
  • 06:11Conclusion

Summary

Automatic Translation

Automatic Translation

The development of neuropathic pain involves pathological changes of spinal cord glial cells. A reliable glial culture system derived from adult spinal cord tissue and designed for studying these cells in vitro is lacking. Therefore, we show here how to establish primary mixed glial cultures from adult mouse spinal cord tissue.

Tags

Primary Mixed Glial CulturesAdult Mouse Spinal CordIn Vitro StudiesNeuropathic PainMultiple SclerosisHBSSPapain DNAseEnzyme DigestionSingle Cell SuspensionAlbumin Ovomucoid InhibitorDNAseCentrifugationMyelin Removal

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