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2D和3D矩阵研究线性侵袭体形成和活性
JoVE Journal
Bioengineering
Author Produced
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JoVE Journal Bioengineering
2D and 3D Matrices to Study Linear Invadosome Formation and Activity
DOI:

12:25 min

June 02, 2017

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Chapters

  • 00:00Title
  • 02:13Preparation of a Collagen-gelatin Mixed Matrix
  • 05:06Immunofluorescence and Linear Invadosome Quantification
  • 07:263D Collagen Invasion Assay
  • 11:19Results
  • 11:33Conclusion

Summary

Automatic Translation

该协议描述了如何制备由明胶和胶原蛋白I组成的2D混合基质,以及3D胶原I插塞来研究线性侵袭体。这些方案允许研究线性侵袭体形成,基质降解活性和原代细胞和癌细胞系的侵袭能力。

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