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Bioengineering
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2D和3D矩阵研究线性侵袭体形成和活性
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
2D and 3D Matrices to Study Linear Invadosome Formation and Activity
Please note that all translations are automatically generated.
Click here for the English version.
2D和3D矩阵研究线性侵袭体形成和活性
DOI:
10.3791/54911-v
•
12:25 min
•
June 02, 2017
•
Julie Di Martino*
1,2
,
Elodie Henriet*
1
,
Zakaria Ezzoukhry
3,4
,
Chandrani Mondal
,
Jose Javier Bravo-Cordero
,
Violaine Moreau
,
Frederic Saltel
1
INSERM U1053
,
2
Icahn School of Medicine at Mount Sinai
,
Tisch Cancer Institute
,
3
Faculté de Médecine, Casablanca
,
Université Mohammed 6 des Sciences de la Santé (UM6SS)
,
4
Laboratoire National de Référence
Chapters
00:00
Title
02:13
Preparation of a Collagen-gelatin Mixed Matrix
05:06
Immunofluorescence and Linear Invadosome Quantification
07:26
3D Collagen Invasion Assay
11:19
Results
11:33
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议描述了如何制备由明胶和胶原蛋白I组成的2D混合基质,以及3D胶原I插塞来研究线性侵袭体。这些方案允许研究线性侵袭体形成,基质降解活性和原代细胞和癌细胞系的侵袭能力。
Tags
Keywords: 2D Matrix
3D Matrix
Linear Invadosome
Extracellular Matrix
Cell-matrix Interaction
Invadosomes
Podosomes
Invadopodia
Metalloproteinases
MMP2
MT1-MMP
Type 1 Collagen Fibrils
Zymography
Gelatin
Mixed Matrix
3D Collagen Invasion Assay
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