Journal
/
Chemistry
/
Insights into the Interactions of Amino Acids and Peptides with Inorganic Materials Using Single-Molecule Force Spectroscopy
JoVE Journal
Chemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Chemistry
Insights into the Interactions of Amino Acids and Peptides with Inorganic Materials Using Single-Molecule Force Spectroscopy

Insights into the Interactions of Amino Acids and Peptides with Inorganic Materials Using Single-Molecule Force Spectroscopy

DOI:
10.3791/54975-v

•

05:44 min

•

March 06, 2017

•

, ,
1Institute of Chemistry and The Center for Nanoscience and Nanotechnology,The Hebrew University of Jerusalem

Chapters

  • 00:05Title
  • 00:40AFM Tip Modification
  • 03:38Single-molecule Force Spectroscopy Measurements
  • 04:41Results: Measuring Single Molecule Adhesion Events
  • 05:18Conclusion

Summary

Automatic Translation

Automatic Translation

Here we present a protocol to measure the force of interactions between a well-defined inorganic surface and either peptides or amino acids by single-molecule force spectroscopy measurements using an atomic force microscope (AFM). The information obtained from the measurement is important to better understand the peptide-inorganic material interphase.

Tags

Single-molecule Force SpectroscopyAmino AcidsPeptidesInorganic MaterialsSurface ChemistryCompositesChordatesAFM CantileversSilane CompoundsFmoc-PEG-NHSPiperidineN-Methyl-2-pyrrolidoneAmino Acid Coupling

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/54975?article_type=v

Preparation of Silica Nanoparticles Through Microwave-assisted Acid-catalysis
check_url/54975?article_type=v

The Synthesis of [Sn10(Si(SiMe3)3)4]2– Using a Metastable Sn(I) Halide Solution Synthesized via a Co-condensation Technique
check_url/54975?article_type=v

Synthesis of Cyclic Polymers and Characterization of Their Diffusive Motion in the Melt State at the Single Molecule Level
check_url/54975?article_type=v

Electrochemical Impedance Spectroscopy as a Tool for Electrochemical Rate Constant Estimation
check_url/54975?article_type=v

Determination of Thermodynamic Properties of Alkaline Earth-liquid Metal Alloys Using the Electromotive Force Technique

Probing the Structure and Dynamics of Interfacial Water with Scanning Tunneling Microscopy and Spectroscopy
check_url/54975?article_type=v

Elemental-sensitive Detection of the Chemistry in Batteries through Soft X-ray Absorption Spectroscopy and Resonant Inelastic X-ray Scattering
check_url/54975?article_type=v

DNA Origami-Mediated Substrate Nanopatterning of Inorganic Structures for Sensing Applications
check_url/54975?article_type=v

Covalent Attachment of Single Molecules for AFM-based Force Spectroscopy
check_url/54975?article_type=v

Atomic Force Microscopy Combined with Infrared Spectroscopy as a Tool to Probe Single Bacterium Chemistry

Read Article