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Brugen af<em> Ex vivo</em> Hele-orgel Imaging og kvantitativ Tissue Histologi at Bestem Bio-distribution af fluorescens-mærkede molekyler
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JoVE Journal Biology
The Use of Ex Vivo Whole-organ Imaging and Quantitative Tissue Histology to Determine the Bio-distribution of Fluorescently Labeled Molecules

Brugen af<em> Ex vivo</em> Hele-orgel Imaging og kvantitativ Tissue Histologi at Bestem Bio-distribution af fluorescens-mærkede molekyler

DOI:
10.3791/54987-v

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08:07 min

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December 24, 2016

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,
1Department of Neurology,The University of Mississippi Medical Center

Chapters

  • 00:05Title
  • 00:46Whole Organ Ex Vivo Fluorescence Imaging
  • 02:00Quantitative Tissue and Standard Histology Preparation
  • 04:22Tissue and Standard Sectioning
  • 05:47Results: Representative Ex Vivo Whole Organ Fluorescence and Quantitative Tissue Histology Analyses
  • 07:15Conclusion

Summary

Automatic Translation

Automatic Translation

Ex vivo hel-organ imaging er en hurtig metode til bestemmelse af de relative koncentrationer af fluorescens mærkede forbindelser inden for og mellem væv eller behandlingsgrupper. Omvendt kvantitativ fluorescens histologi, mens mere arbejdskrævende, giver mulighed for kvantificering af de absolutte væv niveauer af mærkede molekyler.

Tags

Keywords: Ex Vivo Whole-organ ImagingBio-distributionFluorescently Labeled MoleculesProtein-based TherapeuticsTargeting MoleculesAnimal ModelsFluorescent ImagingOCT Embedding

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