/
/
Stillads-understøttet transplantation af islænder i epididymisk fedtpude af diabetiske mus
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:00Title
- 00:09Introduction
- 01:03Cannulation and Perfusion
- 03:55Purification and Handpicking
- 06:30Troubleshooting: Common Bile Duct Distended During Perfusion
- 07:03Troubleshooting: Duodenum Distended During Perfusion
- 07:37Islet Transplantation at EFP Site
- 10:23Results
- 11:29Conclusion
Denne protokol demonstrerer murin islet isolering og såning på en decellulariseret stillads. Stilladsunderstøttede øer blev transplanteret i den epididymiske fedtpude af streptozotocin (STZ) -inducerede diabetiske mus. Islets overlevede på transplantationsstedet og reverserede den hyperglykæmiske tilstand.
