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Drosophila Preparation and Longitudinal Imaging of Heart Function In Vivo Using Optical Coherence Microscopy (OCM)
 

Drosophila Preparation and Longitudinal Imaging of Heart Function In Vivo Using Optical Coherence Microscopy (OCM)

Article DOI: 10.3791/55002-v • 10:13 min • December 12th, 2016
December 12th, 2016 •
1,2, 2,3, 1,2, 3,4, 2,3, 4, 5, 5, 1,2,3
1Bioengineering Program, Lehigh University, 2Center for Photonics and Nanoelectronics, Lehigh University, 3Department of Electrical and Computer Engineering, Lehigh University, 4State Key Laboratory of Software Engineering, Wuhan University, 5Genetics and Aging Research Unit, Department of Neurology, Massachusetts General Hospital and Harvard Medical School

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Summary
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Here, the experimental protocols are described for preparing Drosophila at different developmental stages and performing longitudinal optical imaging of Drosophila heartbeats using a custom optical coherence microscopy (OCM) system. The cardiac morphological and dynamical changes can be quantitatively characterized by analyzing the heart structural and functional parameters from OCM images.

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Keywords: Drosophila Heart Function Optical Coherence Microscopy (OCM) Cardiac Development Longitudinal Imaging In Vivo Metamorphosis Genetic Similarities Vertebrates Optogenetic Pacing Pacemaker Function Larva Mounting Developmental Stage Heart Tube A-scans B-scans Scanner Voltage Transverse Direction
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