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In vitro differentiering af humane CD4 + Foxp3 + Induced Regulatory T-celler (iTregs) fra naive CD4 + T-celler under anvendelse et TGF-β-holdige protokol
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Immunology and Infection
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JoVE Journal Immunology and Infection
In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells (iTregs) from Naïve CD4+ T Cells Using a TGF-β-containing Protocol

In vitro differentiering af humane CD4 + Foxp3 + Induced Regulatory T-celler (iTregs) fra naive CD4 + T-celler under anvendelse et TGF-β-holdige protokol

DOI:
10.3791/55015-v

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08:20 min

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December 30, 2016

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, , ,
1Unit of Computational Medicine, Center for Molecular Medicine, Department of Medicine Solna,Karolinska Institutet, Karolinska University Hospital, & Science for Life Laboratory

Chapters

  • 00:05Title
  • 01:03Peripheral Blood T Cell Isolation
  • 03:43Treg Induction, Plating and Monitoring, and Staining
  • 05:52Results: Representative CD4+FOXP3+ iTreg Analyses
  • 07:25Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokol beskriver den reproducerbare generation og fænotypebestemmelse af humane inducerede regulatoriske T-celler (iTregs) fra naive CD4 + -T-celler in vitro. Forskellige protokoller for Foxp3 induktion muliggøre undersøgelsen af ​​særlige iTreg fænotyper opnået med respektive protokoller.

Tags

In VitroDifferentiationHumanCD4+FOXP3+Induced Regulatory T CellsITregsNaive CD4+ T CellsTGF-βPhenotypeT Cell ImmunologyPBMCMagnetic Bead IsolationBiotin AntibodyAnti-biotin MicrobeadsLS Column

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