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Derivazione delle Culture leptomeningi Explant da Postmortem cervello umano donatori
JoVE Journal
Neuroscience
Author Produced
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JoVE Journal Neuroscience
Derivation of Leptomeninges Explant Cultures from Postmortem Human Brain Donors
DOI:

05:18 min

January 21, 2017

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Chapters

  • 00:00Title
  • 00:51Transferring Meninges to Cutting Dish
  • 01:14Washing Meninges to Remove Blood
  • 01:52Sectioning Meninges under Dissecting Microscope
  • 02:49Transferring Meninges to Culture Plate
  • 03:19Adding Coverslips over Meninges
  • 04:20Representative Results
  • 04:50Conclusion

Summary

Automatic Translation

Il protocollo cultura leptomeningi espianto dal post-mortem cervello umano è un modo tecnicamente robusto e semplice da ricavare fibroblasti meningei fibronectina-positivo entro 6-8 settimane e crioconservazione circa 20-30 milioni di cellule.

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