Journal
/
Bioengineering
/
Ligand Nano-cluster Arrays in a Supported Lipid Bilayer
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Bioengineering
Ligand Nano-cluster Arrays in a Supported Lipid Bilayer

Ligand Nano-cluster Arrays in a Supported Lipid Bilayer

DOI:
10.3791/55060-v

•

10:34 min

•

April 23, 2017

•

, , , ,
1Aix-Marseille Université,CNRS, UMR 7325, CINaM, 2State Key Laboratory of Food Science and Technology,School of Food Science of Jiangnan University

Chapters

  • 00:05Title
  • 00:53Fabrication of Protein Nano-dots
  • 04:31Deposition of Supported Lipid Bilayer
  • 07:39Results: Quantification of Lipid Diffusion and Complementarity of the Nano-dot and SLB Pattern
  • 09:10Conclusion

Summary

Automatic Translation

Automatic Translation

We present a protocol to functionalize glass with nanometric protein patches surrounded by a fluid lipid bilayer. These substrates are compatible with advanced optical microscopy and are expected to serve as platform for cell adhesion and migration studies.

Tags

Ligand Nano-cluster ArraysSupported Lipid BilayerSurface MicroscopyT Cell ActivationAdhesionCell BiologyImmunologyCell TypesOncologySilica BeadsRadio Frequency Magnetron SputteringAluminum CoatingUltrasound

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis

Investigating Receptor-ligand Systems of the Cellulosome with AFM-based Single-molecule Force Spectroscopy

Lipid Bilayer Vesicle Generation Using Microfluidic Jetting

Preparation of Mica Supported Lipid Bilayers for High Resolution Optical Microscopy Imaging

Atomic Force Microscopy Imaging and Force Spectroscopy of Supported Lipid Bilayers

Biomembrane Fabrication by the Solvent-assisted Lipid Bilayer (SALB) Method

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers

Automated Lipid Bilayer Membrane Formation Using a Polydimethylsiloxane Thin Film

Temperature-Controlled Assembly and Characterization of a Droplet Interface Bilayer

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions

Read Article