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在子宫内
电途径研究神经元亚群的兴奋性和单细胞连接
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
In Utero
Electroporation Approaches to Study the Excitability of Neuronal Subpopulations and Single-cell Connectivity
Please note that all translations are automatically generated.
Click here for the English version.
在子宫内
电途径研究神经元亚群的兴奋性和单细胞连接
DOI:
10.3791/55139-v
•
10:49 min
•
February 15, 2017
•
Carlos G. Briz
,
Marta Navarrete
,
José A. Esteban
,
Marta Nieto
1
Department for Molecular and Cellular Biology
,
Centro Nacional de Biotecnología (CNB-CSIC)
,
2
Molecular Neurobiology Department, Centro de Biología Molecular “Severo Ochoa”
,
Consejo Superior de Investigaciones Científicas (CSIC-UAM)
Chapters
00:05
Title
00:48
Injection of DNA and Electroporation
01:52
Sectioning and Staining of Brain Tissue
04:17
Imaging and Analysis
05:42
Electrophysiology
08:29
Results: Representative Firing Pattern of a CAG-GFP Electroporated Pyramidal Neuron
09:39
Conclusion
Summary
Automatic Translation
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Automatic Translation
这份手稿规定,
在子宫内
电(IUE)用来描述在单细胞水平与荧光标记的神经元兴奋性神经元的结构连接协议。组织学是用于表征树突和轴突突起。在急性切片全细胞记录被用来研究兴奋性。
Tags
In Utero Electroporation
Neuronal Subpopulations
Single-cell Connectivity
Developmental Neurobiology
Cognitive Disorders
Sparse Population Of Neurons
Dendrites
Patch Clamp Study
Lateral Ventricle
Cryoprotection
Cryosectioning
Immunohistochemistry
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