/

/

在子宫内电途径研究神经元亚群的兴奋性和单细胞连接

JoVE Journal
Neuroscience

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Neuroscience

In Utero Electroporation Approaches to Study the Excitability of Neuronal Subpopulations and Single-cell Connectivity

Please note that all translations are automatically generated. Click here for the English version.

在子宫内电途径研究神经元亚群的兴奋性和单细胞连接

DOI:

10.3791/55139-v

•

10:49 min

•

February 15, 2017

•

Carlos G. Briz, Marta Navarrete, José A. Esteban, Marta Nieto

¹Department for Molecular and Cellular Biology,Centro Nacional de Biotecnología (CNB-CSIC), ²Molecular Neurobiology Department, Centro de Biología Molecular “Severo Ochoa”,Consejo Superior de Investigaciones Científicas (CSIC-UAM)

Chapters

00:05Title
00:48Injection of DNA and Electroporation
01:52Sectioning and Staining of Brain Tissue
04:17Imaging and Analysis
05:42Electrophysiology
08:29Results: Representative Firing Pattern of a CAG-GFP Electroporated Pyramidal Neuron
09:39Conclusion

Summary

Automatic Translation

这份手稿规定， 在子宫内电（IUE）用来描述在单细胞水平与荧光标记的神经元兴奋性神经元的结构连接协议。组织学是用于表征树突和轴突突起。在急性切片全细胞记录被用来研究兴奋性。

Tags

In Utero Electroporation Neuronal Subpopulations Single-cell Connectivity Developmental Neurobiology Cognitive Disorders Sparse Population Of Neurons Dendrites Patch Clamp Study Lateral Ventricle Cryoprotection Cryosectioning Immunohistochemistry

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

在子宫内的电穿孔其次原发性神经文化为研究基因的功能在皮层神经元的一个子集

在子宫内电穿孔：鼠标控制时空基因Transefection

神经元形态的研究方法：<em>体外</em> RNAi技术在胚胎小鼠大脑皮质的电穿孔

可视化和遗传操作在小鼠大脑皮层和使用海马树突和棘<em>在子宫内</em>电穿孔

外用转基因大鼠通过代<em>在子宫内</em>电穿孔和<em>在体内</em>生物发光筛选

遗传操纵鼠标的发展下丘脑通过<em>在子宫内</em>电穿孔

谷氨酸和GABA快速微离子导入：一个有用的工具，调查突触整合

小脑颗粒神经元的遗传操作<em>体外</em>和<em>在体内</em>为研究神经元形态和迁移

等电脑国家的诱导研究内源性突触活动对神经元兴奋性影响<em>在体内</em

分析树突状形态的列和图层

Read Article