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I Utero Elektroporation Approaches to Undersøgelse ophidselse af neuronale subpopulationer og Single-celle Connectivity
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JoVE Journal Neuroscience
In Utero Electroporation Approaches to Study the Excitability of Neuronal Subpopulations and Single-cell Connectivity

I Utero Elektroporation Approaches to Undersøgelse ophidselse af neuronale subpopulationer og Single-celle Connectivity

DOI:
10.3791/55139-v

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10:49 min

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February 15, 2017

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, , ,
1Department for Molecular and Cellular Biology,Centro Nacional de Biotecnología (CNB-CSIC), 2Molecular Neurobiology Department, Centro de Biología Molecular “Severo Ochoa”,Consejo Superior de Investigaciones Científicas (CSIC-UAM)

Chapters

  • 00:05Title
  • 00:48Injection of DNA and Electroporation
  • 01:52Sectioning and Staining of Brain Tissue
  • 04:17Imaging and Analysis
  • 05:42Electrophysiology
  • 08:29Results: Representative Firing Pattern of a CAG-GFP Electroporated Pyramidal Neuron
  • 09:39Conclusion

Summary

Automatic Translation

Automatic Translation

Dette håndskrift indeholder protokoller, der bruger i livmoderen elektroporation (IUE) til at beskrive den strukturelle konnektivitet af neuroner på encellede niveau og ophidselse af fluorescens-mærkede neuroner. Histologi anvendes til at karakterisere dendritiske og axonale projektioner. Helcelle-optagelse i akutte skiver anvendes til at undersøge ophidselse.

Tags

Keywords: In Utero ElectroporationNeuronal SubpopulationsSingle-cell ConnectivityDevelopmental NeurobiologyCognitive DisordersSparse Population Of NeuronsDendritesPatch Clamp StudyLateral VentricleCryoprotectionCryosectioningImmunohistochemistry

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