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Characterization of Calcification Events Using Live Optical and Electron Microscopy Techniques in a Marine Tubeworm
 

Characterization of Calcification Events Using Live Optical and Electron Microscopy Techniques in a Marine Tubeworm

Article DOI: 10.3791/55164-v • 15:40 min • February 28th, 2017
February 28th, 2017 •
1, 2, 3, 3, 4, 1
1Department of Biological Sciences, Clemson University, 2Department of Marine Biodiversity Research (BioDive), Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 3Advanced Material Research Laboratory (AMRL), Clemson University, 4Swire Institute of Marine Sciences and School of Biological Sciences, The University of Hong Kong

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Summary
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We demonstrate the use of various microscopy methods that are useful in observing the calcification of a tubeworm, Hydroides elegans, as well as locating and characterizing the first calcified material. Live microscopy and electron microscopy are used together to provide functional and material information that are important in studying biomineralization.

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Keywords: Calcification Optical Microscopy Electron Microscopy Marine Tubeworm Intracellular PH Calcium SNARF-1 AM SEM-EDS EBSD FIB TEM
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