Journal
/
Neuroscience
/
Preparation of Horizontal Slices of Adult Mouse Retina for Electrophysiological Studies
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Preparation of Horizontal Slices of Adult Mouse Retina for Electrophysiological Studies

Preparation of Horizontal Slices of Adult Mouse Retina for Electrophysiological Studies

DOI:
10.3791/55173-v

•

07:02 min

•

January 27, 2017

•

,
1Department of Biology,Friedrich-Alexander-University (FAU) Erlangen-Nürnberg

Chapters

  • 00:05Title
  • 00:57Dissection of Mouse Retina
  • 03:12Embedding of Retinal Pieces in Agarose
  • 04:14Mounting of Retinal Pieces
  • 04:58Results: Synaptic Activity Recorded from a Horizontal Cell Body
  • 06:24Conclusion

Summary

Automatic Translation

Automatic Translation

We developed a horizontal slice preparation of the adult mammalian retina with the plane of sectioning parallel to the retinal surface. Dendritic fields of nonradially oriented retinal neurons were not truncated, allowing the study of signal processing with patch-clamp and imaging techniques.

Tags

Keywords: Horizontal SlicesAdult Mouse RetinaElectrophysiological StudiesRetinal NeuronsRibbon SynapsesVisual Information ProcessingHorizontal CellsAmacrine CellsEyeball DissectionCornea RemovalVitreous Body RemovalRetina Isolation

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/55173?article_type=v

Organotypic Culture of Full-thickness Adult Porcine Retina
check_url/55173?article_type=v

In vivo Electroporation of Developing Mouse Retina
check_url/55173?article_type=v

Electrophysiological Characterization of GFP-Expressing Cell Populations in the Intact Retina
check_url/55173?article_type=v

Time-lapse Imaging of Neuroblast Migration in Acute Slices of the Adult Mouse Forebrain
check_url/55173?article_type=v

Preparation of Acute Subventricular Zone Slices for Calcium Imaging
check_url/55173?article_type=v

Dual Electrophysiological Recordings of Synaptically-evoked Astroglial and Neuronal Responses in Acute Hippocampal Slices
check_url/55173?article_type=v

Improved Preparation and Preservation of Hippocampal Mouse Slices for a Very Stable and Reproducible Recording of Long-term Potentiation
check_url/55173?article_type=v

Electrophysiological Recording in the Brain of Intact Adult Zebrafish
check_url/55173?article_type=v

Healthy Brain-pituitary Slices for Electrophysiological Investigations of Pituitary Cells in Teleost Fish
check_url/55173?article_type=v

Assessment of Long-term Depression Induction in Adult Cerebellar Slices

Read Article