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Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX
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JoVE Journal Genetics
Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX

Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX

DOI:
10.3791/55187-v

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10:09 min

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June 27, 2017

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, , ,
1Department of Chemistry and Biomolecular Sciences,University of Ottawa, 2School of Biosciences,The University of Nottingham Malaysia Campus

Chapters

  • 00:05Title
  • 00:49Cloning Genes of Interest in the Multigene Expression System Vector, pMGX
  • 04:11Insert Gene 2 into the pMGX Vector Containing Gene 1, pMGX-yfg1
  • 07:08Results: Multigene Expression in pMGX
  • 09:10Conclusion

Summary

Automatic Translation

Automatic Translation

This study describes methods for the T7-mediated co-expression of multiple genes from a single plasmid in Escherichia coli using the pMGX plasmid system.

Tags

Keywords: Inducible T7 RNA PolymeraseMultigene Expression SystemPMGXSynthetic BiologyBiochemistryProtein ComplexesCo-expressionEscherichia ColiGene CloningRestriction Enzyme DigestionGel ElectrophoresisGel ExtractionLigationBacterial TransformationAntibiotic Selection

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