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Acute and Chronic Models of Hyperglycemia in Zebrafish: A Method to Assess the Impact of Hyperglycemia on Neurogenesis and the Biodistribution of Radiolabeled Molecules
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Chapters
- 00:00Title
- 01:31Establishing a Model of Acute Hyperglycemia in Zebrafish
- 02:59Establishing a Model of Chronic Hyperglycemia in Zebrafish
- 03:41Measuring Blood Glucose Levels in Zebrafish
- 05:00Analyzing Brain Cell Proliferation Following Hyperglycemia
- 06:24Sample Preparation for Immunohistochemistry
- 09:09Microscopy
- 10:05Studying the Impact of Hyperglycemia on Brain Repair Mechanism
- 11:08Imaging the Bio-distribution of Radiolabeled Molecules by PET-Scan
- 12:28Representative Results of [18F]-FDG PT/CT Imaging Distribution in Zebrafish
- 12:48Conclusion
This work describes methods to establish acute and chronic hyperglycemia models in zebrafish. The aim is to investigate the impact of hyperglycemia on physiological processes, such as constitutive and injury-induced neurogenesis. The work also highlights the use of zebrafish to follow radiolabeled molecules (here, [18F]-FDG) using PET/CT.
