Journal
/
Biology
/
MitoCeption: Overførsel isoleret humant MSC Mitokondrier til Glioblastoma Stamceller
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
MitoCeption: Transferring Isolated Human MSC Mitochondria to Glioblastoma Stem Cells

MitoCeption: Overførsel isoleret humant MSC Mitokondrier til Glioblastoma Stamceller

DOI:
10.3791/55245-v

•

11:13 min

•

February 22, 2017

•

, , , , , ,
1Institute for Regenerative Medicine and Biotherapy (IRMB), INSERM U1183,Montpellier University, 2Institute of Neurosciences of Montpellier (INM), INSERM U1051,Montpellier University

Chapters

  • 00:05Title
  • 01:09Seeding of Glioblastoma Stem Cells
  • 03:00Mesenchymal Stem Cells Mitochondria Isolation
  • 06:13Transfer of Isolated MSC Mitochondria to GSCs
  • 07:50Results: Isolation and Transfer of Mitochondria into Mesenchymal Stem Cells
  • 09:39Conclusion

Summary

Automatic Translation

Automatic Translation

Her er en protokol (MitoCeption) præsenterede at overføre mitokondrier, isoleret fra humane mesenkymale stamceller (MSC), til glioblastom stamceller (GSC), med det mål at studere deres biologiske virkninger på GSC metabolisme og funktioner. En lignende protokol kan tilpasses til at overføre mitokondrier mellem andre celletyper.

Tags

Mitochondria TransferMitoCeptionGlioblastoma Stem CellsMesenchymal Stem CellsMetabolismTunneling NanotubesMicrovesiclesVital DyesCell LabelingCell CultureNeurospheresTrypsinDNase ITrypsin InhibitorCell CountingCell SeedingCentrifugationCell IncubationMitochondria Isolation

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/55245?article_type=v

Propagation of Human Embryonic Stem (ES) Cells
check_url/55245?article_type=v

Counting Human Neural Stem Cells
check_url/55245?article_type=v

Passaging Human Neural Stem Cells
check_url/55245?article_type=v

Immunocytochemistry: Human Neural Stem Cells
check_url/55245?article_type=v

Chromatin Immunoprecipitation from Human Embryonic Stem Cells
check_url/55245?article_type=v

Purification of Mitochondria from Yeast Cells
check_url/55245?article_type=v

Freezing and Thawing Human Embryonic Stem Cells
check_url/55245?article_type=v

Differentiation of Embryonic Stem Cells into Oligodendrocyte Precursors
check_url/55245?article_type=v

Multi-parameter Måling af permeabilitetsovergang pore åbning i isolerede Mouse Heart Mitokondrier
check_url/55245?article_type=v

Induktion og analyse af Epithelial til Mesenchymale Transition

Read Article