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Efficient Differentiation of Pluripotent Stem Cells to NKX6-1+ Pancreatic Progenitors
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Developmental Biology
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JoVE Journal Developmental Biology
Efficient Differentiation of Pluripotent Stem Cells to NKX6-1+ Pancreatic Progenitors

Efficient Differentiation of Pluripotent Stem Cells to NKX6-1+ Pancreatic Progenitors

DOI:
10.3791/55265-v

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09:23 min

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March 07, 2017

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,
1Toronto General Research Institute,University Health Network, 2McEwen Centre for Regenerative Medicine,University Health Network, 3Department of Physiology,University of Toronto

Chapters

  • 00:05Title
  • 01:01HESC Differentiation and Flow Cytometry Analysis
  • 01:28Harvesting Cells for Flow Cytometry Analysis
  • 03:17Flow Preparation for Live Staining on Day 3
  • 04:41Flow Preparation for Fixed Staining on Day 13
  • 07:04Results: Schematic of Pancreatic Progenitor Differentiation from hESCs and Supporting Data
  • 08:26Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe a 4-stage protocol to differentiate human embryonic stem cells to NKX6-1+ pancreatic progenitors in vitro. This protocol can be applied to a variety of human pluripotent stem cell lines.

Tags

Keywords: Pluripotent Stem CellsPancreatic ProgenitorsNKX6-1In Vitro DifferentiationPancreas DevelopmentCXCR4CD117PDX1Flow CytometryDefinitive EndodermDiabetes TreatmentDisease Modeling

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