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Immunology and Infection

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JoVE Journal Immunology and Infection
Murine Lymphocyte Labeling by 64Cu-Antibody Receptor Targeting for In Vivo Cell Trafficking by PET/CT
 

Murine Lymphocyte Labeling by 64Cu-Antibody Receptor Targeting for In Vivo Cell Trafficking by PET/CT

Article DOI: 10.3791/55270-v • 11:34 min • April 29th, 2017
April 29th, 2017 •
1, 1, 1, 1, 1, 1,2, 1
1Werner Siemens Imaging Center, Department of Preclinical Imaging and Radiopharmacy, Eberhard Karls University Tübingen, 2Department of Dermatology, Eberhard Karls University Tübingen

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Summary
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Following the preparation of a 64Cu-modified monoclonal antibody binding to a transgenic murine T cell receptor, T cells are radiolabeled in vivo, analyzed for viability, functionality, labeling stability and apoptosis, and adoptively transferred into mice with an airway delayed-type hypersensitivity reaction for non-invasive imaging by positron emission tomography/computed tomography (PET/CT).

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Keywords: Murine Lymphocytes 64Cu-antibody Receptor PET/CT Cell Trafficking Inflammation Cancer Cell-based Therapies Regenerative Medicine Cancer Immunotherapies Cell Migration Cell Labeling Chelator Conjugation Radiolabeling Lymph Nodes Spleen CD4-positive Cells Magnetic Cell Separation
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