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Identifikation af Plasmodesmal lokalisering sekvenser i proteiner i Planta
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Immunology and Infection
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JoVE Journal Immunology and Infection
Identification of Plasmodesmal Localization Sequences in Proteins In Planta

Identifikation af Plasmodesmal lokalisering sekvenser i proteiner i Planta

DOI:
10.3791/55301-v

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08:07 min

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August 15, 2017

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, ,
1Department of Biochemistry and Cell Biology,State University of New York, 2Department of Plant Pathology and Plant-Microbe Biology,Cornell University

Chapters

  • 00:05Title
  • 00:59Plant Growth
  • 01:48Agroinfiltration
  • 04:46Confocal Microscopy
  • 06:03Results: Identification of Plasmodesmal Localization Sequences in Proteins
  • 07:22Conclusion

Summary

Automatic Translation

Automatic Translation

Plante intercellulære forbindelser, plasmodesmata (Pd), spiller en central rolle i plante fysiologi og plante-virus interaktioner. Kritisk til Pd transport sortering signaler, der direkte proteiner til Pd. Vores viden om disse sekvenser er imidlertid stadig i sin vorden. Vi beskriver en strategi for at identificere Pd lokalisering signaler i Pd-målrettet proteiner.

Tags

Keywords: PlasmodesmataLocalization SignalIntercellular TransportProtein TargetingNicotiana BenthamianaFluorescent TaggingAgrobacterium TumefaciensTransient Expression

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