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Analysere Cell Surface Adhesjon Ombygging i Response til Mekanisk Tension Bruke magnetiske kuler
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JoVE Journal Biology
Analyzing Cell Surface Adhesion Remodeling in Response to Mechanical Tension Using Magnetic Beads

Analysere Cell Surface Adhesjon Ombygging i Response til Mekanisk Tension Bruke magnetiske kuler

DOI:
10.3791/55330-v

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07:55 min

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March 08, 2017

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, ,
1Institute for Advanced Biosciences,Centre de recherche UGA – INSERM U1209 – CNRS UMR

Chapters

  • 00:05Title
  • 00:44Ligand Conjugation to Magnetic Beads
  • 02:58Application of Tensional Forces on the Ligand-coated Bead Bound Adhesion Receptors on the Dorsal Surface of Cells
  • 05:49Results: Mechanical Tension Induces Adhesion Maturation
  • 07:03Conclusion

Summary

Automatic Translation

Automatic Translation

Celleoverflatevoksninger er sentrale i mechanotransduction, som de overfører mekanisk spenning og iverksette de signalveier involvert i vev homeostase og utvikling. Her presenterer vi en protokoll for å dissekere de biokjemiske mekanismer som aktiveres i respons til spenning, ved hjelp av ligand-belagt magnetiske mikroperler og kraft søknad til vedheft reseptorer.

Tags

Magnetic BeadsCell Surface AdhesionMechanical TensionBiochemical PathwaysMechano-transductionAdhesion ReceptorsSuperparamagnetic BeadsFibronectinCell AdhesionBSAPoly-D-lysineApotransferrinPBSBovine Serum AlbuminCell CultureAdherent Cells

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