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A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time

A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time

DOI:
10.3791/55351-v

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09:38 min

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February 14, 2017

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, , , , ,
1Transfusion Research Center,Belgian Red Cross-Flanders, 2Faculty of Medicine and Health Sciences,Ghent University, 3Blood Service,Belgian Red Cross-Flanders, 4Department of Public Health and Primary Care,KULeuven – University of Leuven

Chapters

  • 00:05Title
  • 01:01Microfluidics Setup
  • 03:56Blood Sample Collection and Preparation
  • 05:33Perfusion Assay
  • 07:06Results: Representative Platelet Adhesion and Coagulation analyses
  • 08:49Conclusion

Summary

Automatic Translation

Automatic Translation

This paper describes an experimental model of hemostasis that simultaneously measures platelet function and coagulation. Platelet and fibrin fluorescence is measured in real-time, and platelet adhesion rate, coagulation rate, and onset of coagulation are determined. The model is used to determine platelet procoagulant properties under flow in concentrates for transfusion.

Tags

Keywords: Microfluidic Flow ChamberPlatelet TransfusionHemostasisPlatelet DepositionFibrin FormationReal-time Video MicroscopyTransfusion MedicinePlatelet FunctionCoagulationContact PathwayExtrinsic PathwayCollagenBlocking BufferNanopump SoftwareHEPES Buffered SalineMicroscope Stage

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