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Kontrollerbar Ion Channel Expression gennem Inducible Transient transfektion
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Genetics
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JoVE Journal Genetics
Controllable Ion Channel Expression through Inducible Transient Transfection

Kontrollerbar Ion Channel Expression gennem Inducible Transient transfektion

DOI:
10.3791/55370-v

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10:00 min

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February 17, 2017

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, ,
1The Institute for Drug Research (IDR), School of Pharmacy, Faculty of Medicine,The Hebrew University of Jerusalem (HUJI)

Chapters

  • 00:05Title
  • 00:45Transfecting the Plasmid of Interest into Cells
  • 02:27Inducing Gene Expression
  • 03:48Calibrating Timeline of Protein Expression
  • 08:20Results: Response of TRPV1 Activation Increases According to Induction Time as Visualized through Calcium Imaging
  • 09:20Conclusion

Summary

Automatic Translation

Automatic Translation

Studere ionkanaler gennem et heterologt udtrykker system er blevet en central teknik i biomedicinsk forskning. I dette manuskript, præsenterer vi en tid effektiv metode til at opnå tæt kontrolleret ionkanal-ekspression ved at udføre transient transfektion under kontrol af en inducerbar promotor.

Tags

Inducible Transient TransfectionIon Channel ExpressionTREX 293 CellsTRPV1EGFPDoxycyclineElectrophysiologyCalcium ImagingPDL-coated Coverslips/chambersControlled Protein ExpressionHeterologous SystemsReplicable ResultsIon Channel ResearchPharmacology

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