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Detektion og berigelse af Rare Antigen-specifikke B-Celler til analyse af fænotype og funktion
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function
DOI:

09:25 min

February 16, 2017

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Chapters

  • 00:05Title
  • 01:04Human Peripheral Blood Mononuclear Cell Isolation (PBMC)
  • 02:19Cell Staining for Magnetic Nanoparticle-based Enrichment
  • 04:41Magnetic Nanoparticle-based Enrichment
  • 06:51Results: Representative Tetanus-toxoid-binding B Cell Post-enrichment Analyses
  • 08:45Conclusion

Summary

Automatic Translation

En enkel, men effektiv metode, der benytter magnetiske nanopartikler til at opdage og berige antigen-reaktive B-celler til funktionel og fænotypisk analyse beskrives.

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