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Journal / Developmental Biology / Induction of Mesenchymal-Epithelial Transitions…
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Developmental Biology

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JoVE Journal Developmental Biology
Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells
 

Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells

Article DOI: 10.3791/55520-v • 11:42 min • April 7th, 2017
April 7th, 2017 •
1, 1, 1, 1, 2, 3, 4, 1, 1, 5, 2, 4, 1
1Department of Medicine, Duke University, 2Department of Bioengineering, Rice University, 3Department of Molecular Genetics and Microbiology, Duke University, 4Solid Tumor Program and the Duke Prostate Center, Duke University Medical Center, 5Duke University Medical Center

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Summary
Automatic Translation

We present here a cell culture method for inducing mesenchymal-epithelial transitions (MET) in sarcoma cells based on combined ectopic expression of microRNA-200 family members and grainyhead-like 2 (GRHL2). This method is suitable for better understanding the biological impact of phenotypic plasticity on cancer aggressiveness and treatments.

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Keywords: Mesenchymal-epithelial Transition Sarcoma Phenotypic Plasticity Gene Regulatory Networks Therapeutic Interventions HEK293T Cells Transfection Lentivirus Production
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