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Journal / Biochemistry / Lipid Droplet Isolation for Quantitative Mass…
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Biochemistry

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JoVE Journal Biochemistry
Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis
 

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis

Article DOI: 10.3791/55585-v • 10:23 min • April 17th, 2017
April 17th, 2017 •
1, 2, 2, 1
1Heinrich Pette Institute, Leibniz Institute for Experimental Virology, 2Core Facility Mass Spectrometric Proteomics, University Medical Center Hamburg-Eppendorf

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Summary
Automatic Translation

Lipid droplets are important organelles for the replication of several pathogens, including the Hepatitis C Virus (HCV). We describe a method to isolate lipid droplets for quantitative mass spectrometry of associated proteins; it can be used under a variety of conditions, such as virus infection, environmental stress, or drug treatment.

Tags

Keywords: Lipid Droplet Isolation Mass Spectrometry Analysis Quantitative Analysis Hepatitis C Virus SILAC Medium Heavy And Light Labeled Cells Protein Incorporation SDS-PAGE MS Analysis
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