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Electrophysiological Analysis of human Pluripotent Stem Cell-derived Cardiomyocytes (hPSC-CMs) Using Multi-electrode Arrays (MEAs)
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Electrophysiological Analysis of human Pluripotent Stem Cell-derived Cardiomyocytes (hPSC-CMs) Using Multi-electrode Arrays (MEAs)

Electrophysiological Analysis of human Pluripotent Stem Cell-derived Cardiomyocytes (hPSC-CMs) Using Multi-electrode Arrays (MEAs)

DOI:
10.3791/55587-v

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11:13 min

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May 12, 2017

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, , , ,
1Department of Anatomy and Embryology,Leiden University Medical Center, 2Department of Applied Stem Cell Technologies,University of Twente

Chapters

  • 00:05Title
  • 01:13Coating of MEA Chips
  • 03:07hPSC-CMs Dissociation and Plating
  • 05:23Ring Removal and Medium Refreshment
  • 06:17Check Signal Quality and Start Experiment
  • 08:47Results: MEAs Recordings of hPSC-CMs
  • 09:52Conclusion

Summary

Automatic Translation

Automatic Translation

Electrophysiological characterization of cardiomyocytes derived from human Pluripotent Stem Cells (hPSC-CMs) is crucial for cardiac disease modeling and for determining drug responses. This protocol provides the necessary information to dissociate and plate hPSC-CMs on multi-electrode arrays, measure their field potential, and a method for analyzing QT and RR intervals.

Tags

Keywords: Pluripotent Stem Cell-derived CardiomyocytesMulti-electrode ArraysElectrophysiological AnalysisField PotentialDrug ScreeningDissociationCell PlatingFibronectin CoatingPTFE RingLI-BPEL Medium

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