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Immunology and Infection

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JoVE Journal Immunology and Infection
A Non-invasive Way to Isolate and Phenotype Cells from the Conjunctiva
 

A Non-invasive Way to Isolate and Phenotype Cells from the Conjunctiva

Article DOI: 10.3791/55591-v • 07:35 min • July 5th, 2017
July 5th, 2017 •
1, 2, 2, 2,3,4,5, 1
1Lee Kong Chian School of Medicine, Nanyang Technological University, 2Singapore Eye Research Institute, 3Singapore National Eye Center, 4Duke-NUS Medical School, 5Yong Loo Lin School of Medicine

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Summary
Automatic Translation

The exposed normal ocular surface consists of cornea and conjunctiva. Epithelial cells, goblet cells and immune cells are present in the conjunctiva. Here, a non-invasive, technique of impression cytology is described using an impression cytology device and flow cytometry to analyze immune cells in the conjunctiva.

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Keywords: Impression Cytology Ocular Surface Immune Cells Non-invasive Cell Phenotyping Ocular Inflammation Ocular Toxicology Ocular Fibrosis Flow Cytometry Conjunctiva
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