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Differentierande kondrocyter från perifert blod härledda humana inducerade plöripotenta stamceller
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1Department of Orthopedics, Beijing Chao-Yang Hospital,Capital Medical University, 2Key Laboratory of Genomic and Precision Medicine, Beijing Institute of Genomics,Chinese Academy of Sciences, 3Clinical and Translational Research Center of Shanghai First Maternity & Infant Hospital, School of Life Sciences and Technology,Tongji University
Chapters
- 00:05Title
- 00:47Embryoid Body (EB) Formation
- 03:37Cell Pellet Formation and Chondrocyte Differentiation
- 05:42Results: Representative hiPSC-Chon Pellet Characterization
- 06:54Conclusion
Vi presenterar ett protokoll för att generera en kondrogenstamning från humant perifert blod (PB) via inducerade pluripotenta stamceller (iPSCs) med hjälp av en integrationsfri metod som innefattar embryoidkropps (EB) -bildning, expansion av fibroblastiska celler och kondrogeninduktion.
