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Developmental Biology
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从骨髓间充质干细胞制备血浆膜囊泡用于潜在的细胞质替代治疗
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Developmental Biology
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JoVE Journal
Developmental Biology
Preparation of Plasma Membrane Vesicles from Bone Marrow Mesenchymal Stem Cells for Potential Cytoplasm Replacement Therapy
Please note that all translations are automatically generated.
Click here for the English version.
从骨髓间充质干细胞制备血浆膜囊泡用于潜在的细胞质替代治疗
DOI:
10.3791/55741-v
•
09:34 min
•
May 18, 2017
•
Li-qun Xu
,
Mei-jia Lin
,
Yun-pan Li
,
Shuang Li
,
Shao-jun Chen
,
Chi-ju Wei
1
Multidisciplinary Research Center
,
Shantou University
Chapters
00:05
Title
01:10
Plasma Membrane Vesicle (PMV) Generation
03:18
PMV Content Examination by Confocal Microscopy
05:11
PMV Gastrocnemius Muscle Injection
07:12
Results: Representative PMV Imaging and Analyses
08:40
Conclusion
Summary
Automatic Translation
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Automatic Translation
与年龄相关的疾病与细胞质组分中的多种缺陷有关。在这里,我们提出了从骨髓间充质干细胞制备质膜囊泡的方案。这种技术可能被用作细胞质替代疗法的手段,以改善或甚至逆转年龄相关表型。
Tags
Plasma Membrane Vesicles
PMVs
Bone Marrow Mesenchymal Stem Cells
BMSCs
Cytoplasm Replacement Therapy
Mechanical Extrusion
Cell Culture
Trypsin-EDTA
Phase Contrast Microscopy
Mitochondrial Membrane Potential
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