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通过从光响应聚合物纳米载体的siRNA释放的时空控制预测基因沉默

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

Predicting Gene Silencing Through the Spatiotemporal Control of siRNA Release from Photo-responsive Polymeric Nanocarriers

Please note that all translations are automatically generated. Click here for the English version.

通过从光响应聚合物纳米载体的siRNA释放的时空控制预测基因沉默

DOI:

10.3791/55803-v

•

11:53 min

•

July 21, 2017

•

Chad T. Greco, Thomas H. Epps, III², Millicent O. Sullivan

¹Department of Chemical and Biomolecular Engineering,University of Delaware, ²Department of Materials Science and Engineering,University of Delaware

Chapters

00:05Title
00:56Formulation of siRNA Nanocarriers
02:09Measuring siRNA Release Using Gell Electrophoresis
04:57Measuring siRNA Release Using Fluorescence Correlation Spectroscopy (FCS)
06:12Cell Culture and In Vitro siRNA Delivery
08:15Controlling Gene Silencing in a Spatiotemporal Manner
09:35Results: Prediction and Spatiotemporal Control of Gene Silencing
11:07Conclusion

Summary

Automatic Translation

我们提出一种新的方法，使用光响应嵌段共聚物更有效的时空控制基因沉默，没有可检测的脱靶效应。此外，可以使用简单的siRNA释放测定法和简单的动力学建模来预测基因表达的变化。

Tags

Gene Silencing SiRNA Release Photo-responsive Polymeric Nanocarriers Spatiotemporal Control Structure-function Relationships Stimuli-responsive Delivery Kinetic Modeling Gene Expression Control Photo-responsive Polymers N/P Ratio SDS Solution UV Laser Nanocarrier Solution

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