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Bioengineering
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通过从光响应聚合物纳米载体的siRNA释放的时空控制预测基因沉默
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Predicting Gene Silencing Through the Spatiotemporal Control of siRNA Release from Photo-responsive Polymeric Nanocarriers
Please note that all translations are automatically generated.
Click here for the English version.
通过从光响应聚合物纳米载体的siRNA释放的时空控制预测基因沉默
DOI:
10.3791/55803-v
•
11:53 min
•
July 21, 2017
•
Chad T. Greco
,
Thomas H. Epps, III
2
,
Millicent O. Sullivan
1
Department of Chemical and Biomolecular Engineering
,
University of Delaware
,
2
Department of Materials Science and Engineering
,
University of Delaware
Chapters
00:05
Title
00:56
Formulation of siRNA Nanocarriers
02:09
Measuring siRNA Release Using Gell Electrophoresis
04:57
Measuring siRNA Release Using Fluorescence Correlation Spectroscopy (FCS)
06:12
Cell Culture and
In Vitro
siRNA Delivery
08:15
Controlling Gene Silencing in a Spatiotemporal Manner
09:35
Results: Prediction and Spatiotemporal Control of Gene Silencing
11:07
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们提出一种新的方法,使用光响应嵌段共聚物更有效的时空控制基因沉默,没有可检测的脱靶效应。此外,可以使用简单的siRNA释放测定法和简单的动力学建模来预测基因表达的变化。
Tags
Gene Silencing
SiRNA Release
Photo-responsive Polymeric Nanocarriers
Spatiotemporal Control
Structure-function Relationships
Stimuli-responsive Delivery
Kinetic Modeling
Gene Expression Control
Photo-responsive Polymers
N/P Ratio
SDS Solution
UV Laser
Nanocarrier Solution
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