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Easy Manipulation of Architectures in Protein-based Hydrogels for Cell Culture Applications
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Biochemistry
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JoVE Journal Biochemistry
Easy Manipulation of Architectures in Protein-based Hydrogels for Cell Culture Applications

Easy Manipulation of Architectures in Protein-based Hydrogels for Cell Culture Applications

DOI:
10.3791/55813-v

•

08:50 min

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August 04, 2017

•

, ,
1Center for Peptide Pharmaceuticals, Faculty of Natural Science,Ulm University

Chapters

  • 00:05Title
  • 00:38Hydrogel Preparation
  • 01:55Generation of Macroporous Hydrogels Through Freeze-drying, Particle Leaching, and Channel Formation
  • 04:26Hydrogel Visualization
  • 05:25Cell Culture Feasibility
  • 07:14Results: Structural Changes in Hydrogel
  • 08:17Conclusion

Summary

Automatic Translation

Automatic Translation

Different methods to manipulate three-dimensional architecture in protein-based hydrogels are evaluated here with respect to material properties. The macroporous networks are functionalized with a cell-adhesive peptide, and their feasibility in cell culture is evaluated using two different model cell lines.

Tags

Keywords: Protein-based HydrogelsCell Culture ApplicationsMacroporous HydrogelsPore Size3D Cell CultureRGD PeptideCell Adhesive PeptideBSATHPCHydrogel PolymerizationFreeze-dryingLiquid NitrogenFreeze-dryer

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