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Journal
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Medicine
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人类肾脏血管周围间质细胞新型临床分级分离方法
JoVE Journal
Medicine
This content is Free Access.
JoVE Journal
Medicine
A Novel Clinical Grade Isolation Method for Human Kidney Perivascular Stromal Cells
Please note that all translations are automatically generated.
Click here for the English version.
人类肾脏血管周围间质细胞新型临床分级分离方法
DOI:
10.3791/55841-v
•
09:05 min
•
August 07, 2017
•
Daniëlle G. Leuning
,
Ellen Lievers
,
Marlies E.J. Reinders
,
Cees van Kooten
,
Marten A. Engelse
,
Ton J. Rabelink
1
Department of Internal Medicine
,
Leiden University Medical Centre
Chapters
00:05
Title
00:53
Kidney Isolation
03:48
Kidney Perivascular Stromal Cell (kPSC) Culture
05:46
Functional Assessment
06:57
Results: Representative Human kPSC Characterization
07:49
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里我们提出肾血管周围间质细胞 (kPSCs) 基于整个器官灌注与消化酶和 NG2 细胞丰富新颖的临床分级分离和培养方法。使用此方法，就可以获得足够的细胞数量，为细胞疗法。
Tags
Kidney Perivascular Stromal Cells
KPSC
Clinical Grade Isolation Method
Regenerative Medicine
Cell Therapy
Perfusion Tray
Collagenase
DNAse
Cell Suspension
Kidney Cell Isolation
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