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Induction and Diagnosis of Tumors in Drosophila Imaginal Disc Epithelia
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Cancer Research
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JoVE Journal Cancer Research
Induction and Diagnosis of Tumors in Drosophila Imaginal Disc Epithelia

Induction and Diagnosis of Tumors in Drosophila Imaginal Disc Epithelia

DOI:
10.3791/55901-v

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08:14 min

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July 25, 2017

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1Structural Biology Center, National Institute of Genetics and Department of Genetics, School of Life Science,SOKENDAI, 2Graduate School of Media and Governance,Keio University

Chapters

  • 00:05Title
  • 00:56Mosaic Clone Induction
  • 02:21Dissection of Larvae
  • 03:19Isolating and Mounting Imaginal Discs
  • 04:29Diagnosing Neoplastic Phenotypes from Confocal Image
  • 06:17Results: Tumors Induction from IgI-RNAi Expression in the Wing Disc
  • 07:40Conclusion

Summary

Automatic Translation

Automatic Translation

Mosaic clone analysis in Drosophila imaginal disc epithelia is a powerful model system to study the genetic and cellular mechanisms of tumorigenesis. Here we describe a protocol to induce tumors in Drosophila wing imaginal discs using the GAL4-UAS system, and introduce a diagnosis method to classify the tumor phenotypes.

Tags

Keywords: Tumor InductionDrosophilaImaginal DiscsMosaic ClonesTumor DevelopmentHeat-shockFlip-out GAL4Third-instar LarvaeDissectionFixationAntibody Staining

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