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Forbedring af anvendelsen af høj molekylvægt Biotinylated Dextran Amin for Thalamocortical projektion sporing i rotter
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Chapters
- 00:04Title
- 00:42Stereotaxic Injection of Tracer
- 02:54Perfusion and Sectioning
- 04:15Double Fluorescent Staining for BDA and Basic Neural Elements in Cerebral Cortex
- 05:07Results: Injection Site and Typical Neural Labeling with High Molecular Weight BDA in the Somatosensory Cortex
- 06:04Conclusion
Vi præsenterer her, en raffineret protokol for at effektivt afsløre biotinylated dextran Amin (BDA) mærkning med en fluorescerende farvning metode gennem en gensidig neurale vej. Det er egnet til at analysere fine strukturen af BDA mærkning og adskille det fra andre neurale elementer under en Konfokal laser scanning mikroskop.
