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Forbedre anvendelsen av høy molekylvekt Biotinylated dekstran Amin for Thalamocortical projeksjon sporing i rotte
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Improving the Application of High Molecular Weight Biotinylated Dextran Amine for Thalamocortical Projection Tracing in the Rat
DOI:

06:39 min

April 12, 2018

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Chapters

  • 00:04Title
  • 00:42Stereotaxic Injection of Tracer
  • 02:54Perfusion and Sectioning
  • 04:15Double Fluorescent Staining for BDA and Basic Neural Elements in Cerebral Cortex
  • 05:07Results: Injection Site and Typical Neural Labeling with High Molecular Weight BDA in the Somatosensory Cortex
  • 06:04Conclusion

Summary

Automatic Translation

Her presenterer vi en raffinert protokoll for å effektivt avsløre biotinylated dekstran Amin (BDA) merking med et fluorescerende flekker metoden ved hjelp av en gjensidig nevrale sti. Det er egnet for analyse av fine strukturen av BDA merking og skille den fra andre nevrale elementer under en AC confocal laserskanning mikroskop.

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