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Bioengineering

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JoVE Journal Bioengineering
Imaging the Root Hair Morphology of Arabidopsis Seedlings in a Two-layer Microfluidic Platform
 

Imaging the Root Hair Morphology of Arabidopsis Seedlings in a Two-layer Microfluidic Platform

Article DOI: 10.3791/55971-v • 09:23 min • August 15th, 2017
August 15th, 2017 •
1,2, 3, 4, 2,3, 3, 1,2, 1,2
1Bredesen Center for Interdisciplinary Research and Graduate Education, University of Tennessee, 2Bioscience Division and Center for Nanophase Materials Sciences, Oak Ridge national Laboratory, 3Department of Biochemistry and Cellular and Molecular Biology, University of Tennessee, 4Department of Microbiology, University of Tennessee

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Summary
Automatic Translation

This article demonstrates how to culture Arabidopsis thaliana seedlings in a two-layer microfluidic platform that confines the main root and root hairs to a single optical plane. This platform can be used for real-time optical imaging of fine root morphology as well as for high-resolution imaging by other means.

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Keywords: Microfluidic Platform Arabidopsis Seedlings Root Hair Morphology Plant Biology PDMS Seed Sterilization Stratification Plant-based Media Agar
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