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Nanopore DNA Sequencing for Metagenomic Soil Analysis
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Nanopore DNA Sequencing for Metagenomic Soil Analysis

Nanopore DNA Sequencing for Metagenomic Soil Analysis

DOI:
10.3791/55979-v

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07:33 min

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December 14, 2017

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, ,
1Center for Biotechnology Education, Krieger School of Arts and Sciences,Johns Hopkins University

Chapters

  • 00:05Title
  • 00:45Rapid Library Preparation Method
  • 02:06Setup and Sequencing Run
  • 05:36Results: Classroom Application
  • 06:47Conclusion

Summary

Automatic Translation

Automatic Translation

Nanopore technology for sequencing biomolecules has wide applications in the life sciences, including identification of pathogens, food safety monitoring, genomic analysis, metagenomic environmental monitoring, and characterization of bacterial antibiotic resistance. In this article, the procedure for metagenomic soil DNA sequencing for species identification using the nanopore sequencing technology is demonstrated.

Tags

NanoporeDNA SequencingMetagenomicsSoil AnalysisReal-time SequencingBioinformaticsDNA FragmentationLibrary PreparationFlow CellQuality ControlSample Preparation

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