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1Department of Molecular Physiology and Biophysics,University of Virginia School of Medicine, 2Robert M. Berne Cardiovascular Research Center,University of Virginia School of Medicine, 3Department of Hematology,St. Jude Children’s Research Hospital, 4Research Histology Core,University of Virginia School of Medicine
Chapters
- 00:05Title
- 00:49Plating Cells for Vascular Cell co-culture
- 04:14Harvesting Fractions from the VCCC
- 07:21Harvesting and Embedding the VCCC for Transverse Immunofluorescence
- 09:11Results: Myoendothelial Junction Formation in Vascular Cell Co-cultures
- 10:18Conclusion
En celle kultur modell av motstand arterier er beskrevet, slik at dissection signalnettverk trasé i endotelet, glatt muskel, eller mellom endotelet og glatt muskel (myoendothelial krysset). Selektiv bruk av agonister eller protein isolasjon, elektronmikroskop eller immunofluorescence kan utnyttes ved hjelp av denne cellen kultur modellen.
