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1Department of Molecular Physiology and Biophysics,University of Virginia School of Medicine, 2Robert M. Berne Cardiovascular Research Center,University of Virginia School of Medicine, 3Department of Hematology,St. Jude Children’s Research Hospital, 4Research Histology Core,University of Virginia School of Medicine
Chapters
- 00:05Title
- 00:49Plating Cells for Vascular Cell co-culture
- 04:14Harvesting Fractions from the VCCC
- 07:21Harvesting and Embedding the VCCC for Transverse Immunofluorescence
- 09:11Results: Myoendothelial Junction Formation in Vascular Cell Co-cultures
- 10:18Conclusion
En cell kultur modell av motstånd artärer beskrivs, vilket möjliggör dissektion av signalering vägar i endotel, glatt muskulatur, eller mellan endotel och glatt muskulatur (myoendothelial korsningen). Selektiv tillämpning av agonister eller protein isolering, elektronmikroskopi eller immunofluorescens kan utnyttjas med hjälp av denna cell kultur modell.
